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KMID : 0613820170270091040
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Journal of Life Science
2017 Volume.27 No. 9 p.1040 ~ p.1046
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Isolation and Characterization of Six Microorganisms from the Digestive Tract of the Cricket Gryllus bimaculatus
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Kwon Ki-Sang
Lee Eun-Ryeong
Yoo Bo-Kyung
Go Young-Hwa
Shin Hyo-Jung
Choi Ji-Young
Kwon O-Yu
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Abstract
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We describe the isolation and characterization of six different intestinal microorganisms from the digestive tract of the cricket Gryllus bimaculatus. Based on 16S rRNA gene sequences, we obtained six isolates belonging to four different genera: Staphylococcus, Bacillus, Citrobacter, and Proteus. All the isolates were resistant to ampicillin. Ampicillin is an irreversible inhibitor of the enzymeetranspeptidase, which is needed to make bacterial cell walls. None of the isolates were resistant to kanamycin, which binds to the 30S subunit of the bacterial ribosome and then inhibits total protein synthesis. Gram staining was conducted, in addition to morphological classification under a microscope. Four gram- positive isolates and two gram-negative isolates were detected. The gram-positive isolates were GL1 (round shaped, 2 am in diameter), GL2 (rod shaped, 2.5 μm in length), GL3 (rod shaped, 2 μm in length), and GL4 (round shaped, 1.5 μm in diameter). The gram-negative isolates were GL5 (rod shaped, 2 μm in length) and GL6 (rod-shaped, 2.5 μm in length). Notably, two of the isolates, GL2 and GL4, secreted specific extracellular proteins. These were determined by MALDI-TOF-MS spectral analysis to be a 87 kDa collagenase, 56 kDa hypothetical protein, and 200 kDa hypothetical protein. The six isolates in this study could be used for various biotechnological applications and pest management, both in the field and in greenhouse systems. In addition, it would be interesting to determine the relationship between these isolates and their host.
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KEYWORD
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Cricket Gryllus bimaculatus, intestinal microorganisms, MALDI-TOF-MS, 16S rRNA gene
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